بررسی اثر محافظتی کورکومین بر فعالیت آنزیم های سوپراکسید دیسموتاز و کاتالاز در کلیه موش صحرایی مواجهه شده با استامینوفن

Background and purpose: Acetaminophen is one of the most popular analgesic and antipyretic drugs and its overdose can cause severe damage to liver and kidneys in human and animals. Renal dysfunction and acute renal injury can occur without damage in the liver. Unlike kidney damage, mechanisms of liver damage are poorly understood. The aim of present study was to investigate the protective effect of curcumin, derived from Curcuma longa, on acetaminophen-induced kidney damage. Materials and methods: This study was performed in rats that were divided into five groups. Group I as control, group II was i.p. injected with curcumin (200 mg/kg b.w). Group III received DMSO as vehicle control. Group IV was treated with a single dose of acetaminophen (1000 mg/kg b.w, i.p.), and group V that received acetaminophen+Curcumin. After 24 hours, all rats were sacrificed with mild anesthesia. Urea and creatinine levels were measured in the plasma, and the levels of lipid peroxidation (TBARS) and superoxide dismutase (SOD) and catalase (CAT) activity in the kidney and total antioxidant capacity of plasma (TAC) were determined. Data analysis was done using SPSS and the differences between the groups were analyzed applying t-test and Mann Whitney tests at the significance level of 0.05. Results: Administration of acetaminophen caused elevated level of urea and creatinine in plasma and TBARS in kidney. While the activities of SOD and CAT decreased in kidney tissue. Curcumin with acetaminophen decreased the urea, creatinine and TBARS levels significantly but it significantly increased the activity of SOD, CAT, TAC. Conclusion: Our results showed curcumin as the potent protective agent against acetaminophen induced biochemical alterations and oxidative damage in rats. However, further studies are necessary to identify the curcumin’s mechanism of biochemical reaction before clinical application.